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慧诺瑞德发布新品:多光谱光诱导瞬变快速重复荧光仪
点击次数:5702 发布日期:2019-4-24  来源:本站 本站原创,转载请注明出处

 



多光谱光诱导瞬变快速重复荧光仪 LIFT-FRR 是由著名科学家和发明人Zbigniew S. Kolber博士设计的第三代海洋浮游植物光合作用测量系统。

LIFT-FRR用一系列闪光来激发光合并测量叶绿素荧光信号的变化,通过荧光信号的变化来反演光合特性,例如光系统II的有效吸收截面积、光合效率、光合电子传递动力学、非光化学淬灭、受体侧类胡萝卜素淬灭、供体侧P680淬灭、PQ库的大小、瞬时光曲线(Instantaneous light curve, ILC)等。

LIFT-FRR配备445nm、470nm、505nm、540nm和590nm五种激发波长,以及快速重复测量(FRR)模式和连续激发光(CI)模式。在CI模式下还可以使用730nm的远红光。

LIFT-FRR配备软件控制的电动滤光片轮,可以在6个荧光发射通道间切换(默认配置685nm的荧光,另外5个波段可以根据客户需求选配)。

LIFT-FRR配备连续流动样品池,和两通道步进电机驱动的蠕动泵,可以由软件控制泵的速率、方向和时间。

LIFT-FRR的叶绿素浓度检测限为0.1 ug/L。

LIFT-FRR除了可以用比色皿单次测量和连续流动测量外,还可以连接光纤进行外置测量。
 

海洋届最主流的三代浮游植物荧光仪历史进程

1. 1986年Kolber博士和Falkowski教授在Brookhaven国家实验室发明了“泵和探针法”荧光仪,最早实现了光系统功能截面积的测量,但所需测量时间比较长(10-20min);
2. 1992年Kolber博士和Falkowski教授发明了快速重复荧光技术(Fast Repetition Rate, FRR)荧光仪,利用一系列微秒级矩形脉冲电流来产生矩形激发光信号,能够在1秒内测量光合效率、光系统功能截面积和光合电子传递动力学,而且比“泵和探针法”的灵敏度提高了100倍。后来Kolber博士在MBARI和加州大学圣克鲁斯分校工作期间,为FRR荧光仪增加了测量非光化学淬灭、类胡萝卜素淬灭、供体侧淬灭、PO库还原态和瞬时光响应曲线的功能。
3. 2012年,Kolber博士开始将他之前发明的光诱导荧光瞬变技术(Light-Induced FluorescenceTransient, LIFT)导入FRR荧光仪,由此诞生了LIFT-FRR荧光仪。LIFT-FRR允许连续调制驱动电流,使得激发光信号的分辨率达到100ns(时间分辨率提高了10倍。,这极大提高了仪器的测量性能。

涉及这三代荧光仪的核心文献:
1. Kolber, Z. S., J. Zehr, and P. G. Falkowski.1988. Effects of growth irradiance and nitrogen limitation on photosyntheticenergy conversion in Photosystem II. Plant Physiol. 88, 923 929.
2. Kolber, Z. S, K. D. Wyman, and P. G. Falkowski.1990. Natural variability in photosynthetic energy conversion efficiency: afield study in the Gulf of Maine. Limnol. Oceanogr. 35,72 79.
3. Falkowski,P.G., D. Ziemman, Z. S. Kolber, and P. K. Bienfang.1991. Nutrient pumping and phytoplankton response in a subtropical mesoscaleEddy. Nature 352, 55 58.
4. Kolber, Z. S. and P. G. Falkowski.1993. Use of active fluorescence to estimate phytoplankton photosynthesis in situ.Limnol. Oceanogr. 38, 1646 1665.
5. Kolber Z.S., R. T. Barber, K. H. Coale,S. E. Fitzwater, R. M. Greene, K. S. Johnson, S. Lindley, and P. G. Falkowski.1994 Iron limitation of phytoplankton photosynthesis in the Equatorial Pacific Ocean.Nature 371, 145 149.
6. Kolber, Z. S., O. Prasil,and P. G. Falkowski.1998. Measurements of variable chlorophyll fluorescence using fast repetition rate techniques. I. Defining methodology and experimental protocols. Biochem. Biophys.Acta 1367, 88-106
7. Behrenfeld,M. J., and Z. S. Kolber. 1999. Wide spread iron limitation of phytoplankton in the South Pacific Ocean.Science, 283, 840-843
8. Kolber, Z. S, C. L. Van Dover, R. A. Niederman,& P. G. Falkowski. Bacterial photosynthesis in surface waters of the open ocean. 2000. Nature 407, 177-179.
9. Kolber, Z. S., F. G. Plumley, A. S. Lang, J. T. Beatty, R. E. Blankenship, C. L. VanDover,C. Vetriani,M. Koblizek,C. Rathgeber, and P. G. Falkowski. 2001. Contribution of Aerobic Photoheterotrophic Bacteria to the Carbon Cyclein the Ocean.Science 292, 2494-2495
10. Kolber Z. S. Energy Cycle in the Ocean: Powering the Microbial World (2007) Oceanography 20, 82-91, 2007

LIFT-FRR_201904_3


LIFT-FRR技术代表用户

  • 埃克森美孚研究中心(美国)
  • 美国沙漠研究所
  • 澳大利亚国立大学地球科学研究院
  • 俄勒冈州立大学植物学与植物病理系
  • 澳大利亚麦考瑞大学化学与分子生物学系
  • 悉尼理工大学
  • 加拿大不列颠哥伦比亚大学地球与海洋科学系
  • 加拿大蒙特埃里森大学等等
 

LIFT-FRR技术代表文献

  • Brown M, Penta W B, Jones B, Behrenfeld M.The ratio of single-turnover to multiple-turnover fluorescence variespredictably with growth rate and cellular chlorophyll in the green alga Dunaliella tertiolecta.Photosynthesis Research, 2019, 140(1): 65-76.
  • Lewis K M, Arntsen A E,Coupel P,Joy-Warren H, Lowry K E, Matsuoka A, Mills M M, van Dijken G L, Selz V, Arrigo K R. Photoacclimation of Arctic Ocean phytoplankton to shifting light and nutrient limitation. Limnology and Oceanography, 2019, 64(1): 284-301.
  • Read R W, Vuono D C,Neveux I, Staub C, Grzymski J J. Coordinated down regulation of the photosynthetic apparatus as a protective mechanism against UV exposure in the diatom Corethronhystrix. Applied Microbiology and Biotechnology, 2019, 103: 1837-1850..
  • Schuback N, Tortell P D.Diurnal regulation of photosynthetic light absorption, electron transport and carbon fixation in two contrasting oceanic environments. Biogeosciences, 2019, 16: 1381-1399.
  • Hoppe C J M, Schuback N, Semeniuk D,Giesbrecht K, Mol J, Thomas H, Maldonado M T, Rost B, Varela D E, Tortell P D.Resistance of Arctic phytoplankton to ocean acidification and enhanced irradiance.Polar Biology, 2018, 41(3): 399-413.
  • Mills M M, Brown Z W, Laney S R, Ortega-Retuerta E, Lowry K E, van Dijken G L, Arrigo K R. Nitrogen Limitation of the Summer Phytoplankton and Heterotrophic Prokaryote Communities in the Chukchi Sea. Fronties in Marine Science, 2018, 5:362. doi:10.3389/fmars.2018.00362
  • Samanta M, Ellwood M J, Strzepek R F. Zinc isotope fractionation by Emiliania huxleyi cultured across a range of free zinc ion concentrations. Limnology and Oceanography, 2018, 63(2): 660-671.
  • Selz V, Laney S, Arnsten A E, Lewis K M, Lowry K E, Joy-Warren H L, Mills M M, van Dijken G L, Arrigo K R. Ice algal communities in the Chukchi and Beaufort Seas in spring and early summer: Composition, distribution, and coupling with phytoplankton assemblages. Limnologyand Oceanography, 2018, 63(3): 1109-1133.
  • Selz V, Lowry K E, Lewis K M, Joy-Warren H L, van de Poll W, Nirmel S,Tong A, Arrigo K R. Distribution of Phaeocystis antarctica-dominated sea ice algal communities and their potential to seed phytoplankton across the western Antarctic Peninsula in spring. Marine Ecology Progress Series,2018, 586: 91-112.
  • HoppeC J M, Schuback N, Semeniuk D M,Maldonado M T, Rost B. Functional Redundancy Facilitates Resilience of Subarctic Phytoplankton Assemblages toward Ocean Acidification and High Irradiance.Frontiersin Marine Science, 2017, 4:229. doi:10.3389/fmars.2017.00229
  • Hussherr R, Levasseur M, Lizotte M, Tremblay J-E, Mol J, Thomas H, Gosselin M, Starr M,Miller L A, Jarnikova T, Schuback N, Mucci A. Impact of ocean acidification on Arctic phytoplankton blooms and dimethylsulfide concentration under simulated ice-free and under-ice conditions. Biogeosciences, 2017, 14: 2407-2427.
  • Levin R A, Suggett D J, Nitschke M R, van Oppen M JH, Steinberg P D. Expanding the Symbiodinium(Dinophyceae, Suessiales) Toolkit Through Protoplast Technology. Journalof Eukaryotic Microbiology, 2017, 64(5): 588-597.
  • Schuback N, Hoppe C J M, Tremblay J-E, Maldonado M T, Tortell P D. Primary productivity and the coupling of photosynthetic electron transport and carbon fixation in the Arctic Ocean. Limnology and Oceanography, 2017, 62(3): 898-921.
  • Shilova I N, Mills M M, Robidart J C,Turk-Kubo K A, Bjorkman K M, Kolber Z, Rapp I, van Dijken G L, Church M J, Arrigo K R, Achterberg E P, Zehr J P. Differential effects of nitrate, ammonium, and urea as N sources for microbial communities in the North Pacific Ocean. Limnology and Oceanography, 2017, 62(6): 2550-2574.
  • SzaboM, Larkum A WD, Suggett D J, Vass I, Sass L, Osmond B, Zavafer A,Ralph P J, Chow W S. Non-intrusive Assessment of Photosystem II and PhotosystemI in Whole Coral Tissues. Frontiers in Marine Science, 2017, 4: 269. doi:10.3389/fmars.2017.00269
  • Schuback N, Flecken M, Maldonado M T, Tortell P D. Diurnal variation in the coupling of photosynthetic electron transport and carbon fixation in iron-limited phytoplankton in the NE subarctic Pacific. Biogeosciences, 2016, 13: 1019-1035.
 

慧诺瑞德(北京)科技有限公司(PhenoTrait)技术团队具有近20年叶绿素荧光特别是藻类叶绿素荧光经验,是Kolber博士的密切合作伙伴,将与Kolber博士一起为您提供高质量产品和专业服务。

发布者:慧诺瑞德(北京)科技有限公司
联系电话:010-62925490
E-mail:info@phenotrait.com


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